biotinylated exosomal marker membrane protein antibody  (Absolute Biotech Inc)

Journal: Translational Vision Science & Technology

Article Title: Aqueous Humor Exosomal Membrane Proteins: Decoding Pathogenic Molecular Signatures in Retinitis Pigmentosa

doi: 10.1167/tvst.14.12.15

Figure Lengend Snippet: EVArray-identified AH exosomal membrane proteins from RPs and controls. ( a ) PLS-DA plot of 21 samples (absolute expression values). ( b ) PLS-DA plot of 21 samples (relative expression values). ( c ) Volcano plot of absolute quantification data. ( d ) Volcano plot of the relative quantification metrics. Red and blue dots represent proteins with an absolute value of log 2 (FC) ≥0.26 and adjusted P < 0.05; gray dots indicate proteins that failed to pass these conditions. ( e ) Boxplots of upregulated and downregulated AH exosomal membrane protein expression (relative quantification). O range indicates RP, and blue indicates control. FC, fold change; RFU, relative fluorescence units. * P < 0.05, ** P < 0.01, **** P < 0.0001.

Article Snippet: Following three sequential wash cycles with PBS (200 μL per wash), detection was initiated by the addition of 150 μL biotinylated exosomal marker membrane protein antibody (LifeSpan BioSciences, Seattle, WA, USA; 1:1000–1:1500 dilution range) with 60-minute incubation at 25°C.

Techniques: Membrane, Expressing, Quantitative Proteomics, Control, Fluorescence

Journal: Translational Vision Science & Technology

Article Title: Aqueous Humor Exosomal Membrane Proteins: Decoding Pathogenic Molecular Signatures in Retinitis Pigmentosa

doi: 10.1167/tvst.14.12.15

Figure Lengend Snippet: ROC-AUC curves of differentially expressed exosomal membrane proteins in the AH of RP. ( a ) ROC-AUC curves of downregulated exosomal membrane proteins in the AH of patients with RP. ( b ) ROC-AUC curves of upregulated exosomal membrane proteins in the AH of patients with RP.

Article Snippet: Following three sequential wash cycles with PBS (200 μL per wash), detection was initiated by the addition of 150 μL biotinylated exosomal marker membrane protein antibody (LifeSpan BioSciences, Seattle, WA, USA; 1:1000–1:1500 dilution range) with 60-minute incubation at 25°C.

Techniques: Membrane

Journal: Translational Vision Science & Technology

Article Title: Aqueous Humor Exosomal Membrane Proteins: Decoding Pathogenic Molecular Signatures in Retinitis Pigmentosa

doi: 10.1167/tvst.14.12.15

Figure Lengend Snippet: Single-cell tracing of differentially expressed exosomal membrane proteins in AH. ( a ) Schematic representation of cellular origin inference through data integration. Created in BioRender ( https://BioRender.com/guvbktb ). ( b ) Sankey diagram mapping the tissue-specific cellular provenance of differentially expressed RP-relevant AH exosomal membrane proteins. c, corneal; RPE, retinal pigment epithelium; TM, trabecular meshwork.

Article Snippet: Following three sequential wash cycles with PBS (200 μL per wash), detection was initiated by the addition of 150 μL biotinylated exosomal marker membrane protein antibody (LifeSpan BioSciences, Seattle, WA, USA; 1:1000–1:1500 dilution range) with 60-minute incubation at 25°C.

Techniques: Membrane

Journal: Translational Vision Science & Technology

Article Title: Aqueous Humor Exosomal Membrane Proteins: Decoding Pathogenic Molecular Signatures in Retinitis Pigmentosa

doi: 10.1167/tvst.14.12.15

Figure Lengend Snippet: Chord diagram maps RP-relevant AH exosomal membrane protein–GO term associations. FC, fold change.

Article Snippet: Following three sequential wash cycles with PBS (200 μL per wash), detection was initiated by the addition of 150 μL biotinylated exosomal marker membrane protein antibody (LifeSpan BioSciences, Seattle, WA, USA; 1:1000–1:1500 dilution range) with 60-minute incubation at 25°C.

Techniques: Membrane